Research activity

In addition to having a major role in the education of dental students, faculty of the department is one of the most active basic science departments at the Faculty of Dentistry in terms of research activity. The Department has a strong interest in dental research and the efforts are potentiated by research interactions with both preclinical departments and many clinical departments and subspecialties. This affords let students not only have excellence in academic preparation but also have diverse research opportunities. To support research activity, well equipped laboratories have been set up at the department and research unit. Such research facilities as high-resolution scanning electron microscopy; fluorescence microscopy; immunocytochemistry; electrophoresis; Polymerase Chain Reaction; an anaerobic culture system; high-speed ultracentrifuges, lyophilizers, spectrophotometers, a high-performance liquid chromatography system as well as Cell and tissue culture laboratory is available.

 

Research Interests
• Caries research focusing on risk factors and prevention program in children
• Immunopathogenesis of periodontal diseases
• Development of products, i.e., herb-containing products, used as adjunct methods for prevention and treatment of oral infections
• Diagnostic methods, i.e., Oral hygiene screening test kit, Application of ELISA technique for determining salivary IgA
• Pulp Biology, focusing on Metal Toxicity

 

Publications (1999-2011)

• Amornchat C, Junyaprasert V, Tandhachoon K, Nakee S, Muadcheingka T. The antibacterial properties of a herbal toothpast : an in vitro and in vivo study. J Hlth Sci. 1999; 8: 202-209.

• Chidchungchai W, Kuphasuk Y, Tanakun S, Thaweboon S. Reduction of Streptococcus mutans in high caries-risk children by chlorhexidine varnish. J Dent Assoc Thai 1999; 49 : 86-97.

• Chitchumnong P, Kraivaphan P, Triratana T, Amornchat C, Junyaprasert V. Effect of a herbal sodium bicarbonate dentifrice on plaque and gingivitis : A six month clinical study. Mahidol J 1999; 19: 42-48.
• Reynolds DN, Keeling KL, Molestina R, Srisatjaluk R, Ehringer W, Justus DE, Kotwal GJ. Heparin binding activity of vaccinia virus protein confers additional properties of uptake by mast cells and attachment to endothelial cells. In :Jameel S. and Villarreal L. (eds.) Advances in animal virology. Oxford and IBN Publishing, New Delhi, India 1999; 339-342.
• Srisatjaluk R, Doyle RJ, Justus DE. Outer membrane vesicles of Porphyromonas gingivalis inhibit IFN-g-mediated MHC class II expression by human vascular endothelial cells. Microb Pathog. 1999; 27:81-91.
• Chuckpaiwong S, Boonyasinha K, Thaweboon B, Thaweboon S, Yudtasarnprasit S. Study of microbiological risk factor of dental caries of children in rural remote area. Mahidol Dent J 2000 ; 20 : 105-115.
• Chuckpaiwong S, Boonyasinha K, Thaweboon B, Thaweboon S, Yudtasarnprasit S. Study distribution of mutans Streptococci and lactobacilli in children in rural remote area. Mahidol Dent J 2000 ; 20: 123-132 .
• Chuckpaiwong S, Thaweboon S, Chunhabundit P, Thaweboon B, Suppukpatana P. Effect of low level laser irradiation on human gingival and pulpal fibroblasts. Mahidol J 2000; 7 : 97-100.

• Chuckpaiwong S, Thaweboon B, Thaweboon S, Rukprasitkul S. Sporicidal efficacy of peracetic acid and glutaraldehyde. Mahidol Dent J 2000 ; 20: 89-94.

• Smith SA, Mullin NP, Parkinson J, Shchelkunov SN, Totmenin AV, Loparev VN, Srisatjaluk R, Reynolds DN, Keeling KL, Justus DE, Barlow PN, Kotwal GJ. Conserved surface-exposed K/R-X-K/R Motifs and net positive charge on poxvirus complement control proteins serve as putative heparin binding sites and contribute to inhibition of molecular interactions with human endothelial cells: a novel mechanism for evasion of host defense. J. Virol. 2000; 74:5659-5666.
• Thaweboon S, Chunhabundit P, Surarit S, Swasdison S. Effect of lead on human dental pulp cells in vitro. J Dent Res 2001; 80 (Special Issue): 577.
• Thaweboon S, Thaweboon B. Effect of Phyllanthus emblica Linn. on adherence of Candida albicans. J Dent Res 2001; 80 (Special Issue): 577.

• Thaweboon S, Thaweboon B, Buajeeb W. Effect of Centella asiatica on Candida albicans and treatment of oral candidosis. Mahidol Dent J 2001; 21 : 53-60.

• Thaweboon S, Thaweboon B, Buajeeb W. Effect of plant extracts on the adherence of Candida albicans to buccal epithelial cells. Mahidol Dent J 2001; 21: 61-69.

• Thaweboon B, Thaweboon S, Suppakpatana P. Cytotoxicity of oral bacteria on human pulpal fibroblast
culture. J Dent Res 2001; 80 (Special Issue): 577.

• Timpawat S, Amornchat C, Trisuwan W. Bacterial coronal leakage after obturation with three root canal sealers. J Endodon 2001; 27: 36-39.
• Srisatjaluk R, Kotwal GJ, Hunt LA, Doyle RJ, Justus DE. Modulation of gamma interferon-induced MHC class II gene expression by Porphyromonas gingivalis membrane vesicles. Infect Immun. 2002, 70:1185-1192.

• Thaweboon S, Chunhabundit P, Sararit R, Swasdison S. Effect of lead on the proliferation, protein production and osteocalcin secretion in human dental pulp cells in vitro. Southeast Asian J Trop Med Public Health 2002; 33: 654-666.

• Thaweboon S, Thaweboon B, Suppukpatana P. Effect of dental unit ozonator on microbial reduction and colonization. Mahidol Dent J 2002; 22.

• Amornchat C, Rassameemasmaung S, Sripairojthikoon V, Swasdison S. Invasion of Porphyromonas gingivalis into human gingival fibroblasts in vitro. J Int Acad Periodont 2003; 54:98-105.
• Thaweboon S, Thaweboon B, Chunhabundit P, Suppukpatana P. Effect of fluoride on human dental pulp cells in vitro. Southeast Asian J Trop Med Public
Health 2003; 34:915-8.
• Amornchat C, Kraivaphun P, Triratana T. Investigation of a Triclosan/Copolymer dentrifice on plaque viability and Triclosan retention in dental plaque in Thai population. Mahidol Dent J 2004; 24:103-12.
• Choonharuangdej S. CHROMagar Candida: A simply effective differential medium for Candida albicans. Mahidol Dent J. 2004; 24:65-72.
• Choonharuangdej S, Amornchat C, Tandhachoon K. Salivary immunoglobulin A (IgA) quantitation by ELISA. Mahidol Dent J. 2004; 24:57-64.
• Tantivanich S., Laohaphand P, Thaweboon S, et al. Prevalence of cytomegalovirus, human herpesvirus-6 and Epstein-Barr virus in periodontitis patients and healthy subjects in the Thai population. Southeast Asian J Trop Med Public Health 2004: 35:635-638.

• Thaweboon S, Thaweboon B. Effect of xylitol chewing gum on mutans streptococci and lactobacilli in saliva and dental plaque. Southeast Asian J Trop Med Public Health 2004;35: 1024-7.

• Thaweboon S, Thaweboon B, Nakornchai S, Soo-Ampon S, Chuckpaiwong S. Mutans streptococci transmission from mothers or caretakers to their children in rural remote area of Thailand. Mahidol Dent J 2004; 24:139-145.

• Thaweboon S, Thaweboon B, Soo-ampon S, Chuckpaiwong S. Salivary mutans streptococci and lactobacilli of children in different communities in rural remote area of Thailand. Mahidol Dent J 2004; 24:51-6. (Thai)

• Chailertvanit P, Thaweboon S, Sooksuntisakoonchai N, Ekphisutsunthorn S. In vitro cytotoxicity test of four root canal sealers to human pulpal cells. J Dent Assoc Thai 2005; 55: 178-85.

• Thaweboon S, Thaweboon B, Choonharuangdej S, Chunhabandit P, Suppakpatana P. Induction of type I collagen and osteocalcin in human dental pulp cells by retinoic acid. Southeast Asian J Trop Med Public Health 2005: 1066-1069.

• Thaweboon S, Thaweboon B. Soo-Ampon S., Soo-Ampon M. Salivary mutans streptococci and lactobacilli after self arresting caries treatment. Southeast Asian J Trop Med Public Health 2005: 756-768

• Thaweboon S, Thaweboon B, Soo-Ampon S, Soo-Ampon M. Salivary mutans streptococci after self arresting caries treatment. Southeast Asian J Trop Med Public Health 2005; 35: 1022-5.

• Thaweboon S, Thaweboon B, Veerapradist W. Lead in saliva and its relationship to blood in the residents of Klity village. Southeast Asian J Trop Med Public Health 2005; 36: 1576-8.

• Amornchat C, Kraivaphan P, Dhanabhumi C, Tandhachoon K, Trirattana T, Choonharuangdej S. Effect of Cha-em Thai mouthwash on salivary levels of mutans streptococci and tolal IgA. Southeast Asian J Trop Med Public Health 2006;3:528-531.
• Kraivaphan P, Amornchat C, Triratana T, Leethochawalit Clinical effect of a triclosan containing dentifrice on gingivitis during pregnancy and post-partum. Southeast Asian J Trop Med Public Health 2006;4:1-5.
• Nakornchai S, Thaweboon S. Factors related to early childhood caries. Thai J Dent Public Health 2006; 11: 15-24.

• Palananthana M, Pachimsawat P, Thaweboon S. Microbial finding of reused laminated film pouches stored in closed cabinet for 4 months. Mahidol Dent J 2006; 26: 179-86.

• Suddhasthira T, Thaweboon S, Dendoung N, Thaweboon B, Dechkunakorn S. Antimicrobial activity of Cratoxylum formosum on Streptococcus mutans. Southeast Asian J Trop Med Public Health 2006; 37: 823-7.

• Thanakun S, Thaweboon S, Okuma N. The prevalence and intra-oral distribution of Candida albicansinfection in patients with oral lichen planus. Mahidol Dent J 2006; 26: 197-205.

• Thaweboon S, Nakornchai S. Control of cariogenic bacteria. Thai J Dent Public Health 2006; 11: 25-30.

• Thaweboon B, Thaweboon S, Choonharuangdej S, Suppakpatana P. Effects of sonicated Prevotella intermediaFusobacterium necleatum and Lactobacillus casei extracts on interleukin-8 production by human dental pulp cells. Southeast Asian J Trop Med Public Health 2006; 37: 523-7.

• Thaweboon B, Thaweboon S, Sopavanit C, Kasetsuwan R. A modified Dip-slide test for microbiological risk in caries assessment. Southeast Asian J Trop Med Public Health 2006; 37: 400-4.

• Visuttiwattanakorn S, Srithavaj T, Thaweboon S. Evaluation of microflora around extraoral per-implant percutaneous tissues in a group of Thai patients. Mahidol Dent J 2006; 26: 281-8.

• Lapirattanakul J, Nakano K, Nomura R, Hamada S, Nakagawa I, Ooshima T. Demonstration of mother-to-child transmission of Streptococcus mutans using multilocus sequence typing.Caries Res 2008;42:466-74.

• Nakano K, Nomura R, Nemoto H, Lapirattanakul J, Taniguchi N, Gronroos L, Alaluusua S, Ooshima T. Protein antigen in serotype k Streptococcus mutans clinical isolates.J Dent Res2008;87:964-8.
• Thaweboon S, Srithavaj T, Thaweboon B, Choonharuangdej S. Oral colonization of Candida species in patients receiving radiotherapy in head and neck area. Quintessence Int J 2008; 39:52-6.
 Thaweboon S, Thaweboon B, Nakornchai S, Jitmaitree S. Salivary secretory IgA, pH, flow rates, mutans streptococci and Candida in children with rampant caries. Southeast Asian J Trop Med Public Health 2008; 39:893-9.
• Lapirattanakul J , Nakano K, Nomura R, Nemoto H, Kojima A, Senawongse P, et al. Detection of serotype k Streptococcus mutans in Thai subjects. Oral Microbiol Immunol. 2009;24:431-3.
• Lapirattanakul J . Serotypes of Streptococcus mutans. J Dent Assoc Thai 2009;59:222-32.
• Nomura R, Nakano K, Taniguchi N, Lapirattanakul J, Nemoto H, Gr?nroos L, et al. Molecular and clinical analyses of the gene encoding the collagen-binding adhesin of Streptococcus mutans. J Med Microbiol. 2009 58:469-75.
• Thaweboon S, Nakornchai S, Miyake Y, Yanagisawa T, Thaweboon B, Soo-Ampon S, Lexomboon D. Remineralization of enamel subsurface lesions by xylitol chewing gum containing funoran and calcium hydrogenphosphate. Southeast Asian J Trop Med Public Health 2009;40:345-53.
• Thaweboon S, Thaweboon B.In vitro antimicrobial activity of Ocimum americanum L. essential oil against oral microorganisms.Southeast Asian J Trop Med Public Health 2009;40:1025-33.
• Nakano K, Nomura R, Taniguchi N, Lapirattanakul J, Kojima A, Naka S, et al. Molecular characterization of Streptococcus mutans strains containing the cnm gene encoding a collagen-binding adhesion. Arch Oral Biol. 2010;55:34-9.
• Suwatanapongched P, Surarit R, Srisatjaluk R, Offenbacher S. Translocation of Porphyromonas gingivalis infected monocytes and associated cellular responses. Asian Pac J allergy Immunol 2010; 28:192-9.
• Thaweboon B, Laohapand P, Amornchat C, Matsuyama J, Sato T, Nunez PP, Uematsu H, Hoshino E. Host beta-globin gene fragments in crevicular fluid as a biomarker in periodontal health and disease. J Periodontal Res. 2010; 45: 38-44.
• Thaweboon S, Poomsawat S, Thaweboon B. Effect of lead on IL-8 production and cell proliferation in human oral keratinocytes. APJTM 2010; 3: 475-8.
• Thaweboon S, Thaweboon B. Inhibition of Candida adhesion to denture acrylic by Boesenbergia pandurata. APJTM 2010; 3: 272-5.
• Yuyuen S, Srisatjaluk R. Antibacterial efficiency of 3Mix and modified 3Mix against oral pathogens.Mahidol Dent J 2010; 30:91-102.

• Thaweboon B, Thaweboon S. Effect of Phyllanthus emblica Linn. On candida adhesion to oral epithelium and denture acrylic. APJTM 2011: 41-45.